tgfβ  (R&D Systems)

Journal: Cell Reports Medicine

Article Title: ACVR2A attenuation impacts lactate production and hyperglycolytic conditions attracting regulatory T cells in hepatocellular carcinoma

doi: 10.1016/j.xcrm.2025.102038

Figure Lengend Snippet:

Article Snippet: recombinant human/mouse/rat activin A PLUS protein , MBL , Cat# QK005-0100.

Techniques: Staining, Purification, Recombinant, Modification, Transfection, Functional Assay, Antibody Labeling, Lactate Assay, Glucose Assay, Blocking Assay, Western Blot, Reverse Transcription, Cell Culture, Bicinchoninic Acid Protein Assay, RNA Sequencing, Gene Expression, Plasmid Preparation, shRNA, Software, Real-time Polymerase Chain Reaction, Filtration

Journal: Cell reports

Article Title: Combinatorial genetic engineering strategy for immune protection of stem cell-derived beta cells by chimeric antigen receptor regulatory T cells

doi: 10.1016/j.celrep.2024.114994

Figure Lengend Snippet:

Article Snippet: 3D sphere formation was performed for 48 h followed by induction of definitive endoderm differentiation using d1 media [RPMI containing 0.2% FBS, 1:5,000 ITS (Gibco #41400–045), 100 ng/mL Activin A (R&D Systems #338-AC-01M), and 3 μM CHIR99021 (STEMCELL Technologies #72054)].

Techniques: Virus, Recombinant, Enzyme-linked Immunosorbent Assay, Isolation, Sequencing, Plasmid Preparation, Luciferase, Software